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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ins1 Polyclonal antibody

Ins1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28609-1-AP

Synonyms

Ins 1, Ins1, Ins2 rs1, insulin I, insulin1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ins1 Polyclonal antibody (28609-1-AP)

    WB analysis using 28609-1-AP

    Rat pancreas tissue were subjected to SDS PAGE followed by western blot with 28609-1-AP (insulin1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using Ins1 Polyclonal antibody (28609-1-AP)

    IHC staining of rat pancreas using 28609-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 28609-1-AP (insulin1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using Ins1 Polyclonal antibody (28609-1-AP)

    IHC staining of rat pancreas using 28609-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 28609-1-AP (insulin1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Ins1 Polyclonal antibody (28609-1-AP)

    IHC staining of mouse pancreas using 28609-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28609-1-AP (insulin1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Ins1 Polyclonal antibody (28609-1-AP)

    IHC staining of mouse pancreas using 28609-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28609-1-AP (insulin1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inrat pancreas tissue
Positive IHC detected inrat pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28609-1-AP targets Ins1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ins1 fusion protein Ag29989 相同性解析による交差性が予測される生物種
Full Name insulin I
Calculated molecular weight 12 kDa
Observed molecular weight12 kDa
GenBank accession numberNM_008386
Gene symbol Ins1
Gene ID (NCBI) 16333
RRIDAB_2918180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.

Protocols

Product Specific Protocols
WB protocol for Ins1 antibody 28609-1-APDownload protocol
IHC protocol for Ins1 antibody 28609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols