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Validation Data Gallery

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  • Western Blot (WB) analysis of rat pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    WB analysis of rat pancreas using 83867-1-RR (same clone as 83867-1-PBS)

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 83867-1-RR (insulin1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    IHC staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    IHC staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    IHC staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    IF Staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using insulin1 antibody (83867-1-RR, Clone: 240798G4, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).SST-Specific antibody (84351-1-RR, Clone: 240816D9,red ) at dilution of 1:400 and FlexAble CoraLite® Plus 594 Antibody Labeling Kit for Rabbit IgG(KFA009), Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    IF Staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    IF Staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Ins1 Recombinant antibody (83867-1-RR)

    IF Staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83867-1-RR

    Affinity and Kinetic Characterization of 83867-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83867-1-RR against Mouse Ins1 were performed. The affinity constant is 0.21 nM.

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Mouse Ins1 Recombinant antibody, PBS Only

Ins1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83867-1-PBS
Clone No.240798G4

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Insulin, insulin1, insulin I, Ins2 rs1, Ins-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83867-1-PBS targets Ins1 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Ins1 fusion protein Ag29988 相同性解析による交差性が予測される生物種
Full Name insulin I
Calculated molecular weight 12 kDa
Observed molecular weight12 kDa
GenBank accession numberNM_008386
Gene Symbol Ins1
Gene ID (NCBI) 16333
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01325
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Insulin 1 is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.