Integrin alpha 1 Polyclonal antibody

Integrin alpha 1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29042-1-AP

Synonyms

ITGA1, VLA 1, Laminin and collagen receptor, integrin, alpha 1, CD49a


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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, rat lung tissue
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

29042-1-AP targets Integrin alpha 1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin alpha 1 fusion protein Ag30613 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 1
Calculated molecular weight 1179 aa, 131 kDa
Observed molecular weight180 kDa
GenBank accession numberBC137121
Gene symbol Integrin alpha 1
Gene ID (NCBI) 3672
RRIDAB_2881238
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

Protocols

Product Specific Protocols
WB protocol for Integrin alpha 1 antibody 29042-1-APDownload protocol
IHC protocol for Integrin alpha 1 antibody 29042-1-APDownload protocol
IF protocol for Integrin alpha 1 antibody 29042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Development

TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration

Authors - Binbin Li
humanWB

PeerJ

Production of recombinant human epidermal growth factor fused with HaloTag protein and characterisation of its biological functions

Authors - Mengru Bai