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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Integrin alpha 1 Monoclonal antibody

Integrin alpha 1 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1C4F2

Cat no : 68211-1-Ig

Synonyms

ITGA1, Laminin and collagen receptor, integrin, alpha 1, CD49a, CD49 antigen-like family member A

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Integrin alpha-1 Monoclonal antibody (68211-1-Ig)

    WB analysis of HepG2 using 68211-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68211-1-Ig (Integrin alpha-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Integrin alpha-1 Monoclonal antibody (68211-1-Ig)

    WB analysis of human placenta using 68211-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68211-1-Ig (Integrin alpha-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Integrin alpha 1 Monoclonal antibody (68211-1-Ig)

    IF Staining of human placenta using 68211-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Integrin alpha 1 antibody (68211-1-Ig, Clone: 1C4F2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Integrin alpha 1 Monoclonal antibody (68211-1-Ig)

    IF Staining of human placenta using 68211-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Integrin alpha 1 antibody (68211-1-Ig, Clone: 1C4F2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, human placenta tissue
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68211-1-Ig targets Integrin alpha 1 in WB, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Integrin alpha 1 fusion protein Ag30631 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 1
Calculated molecular weight 1179 aa, 131 kDa
Observed molecular weight180 kDa
GenBank accession numberBC137121
Gene symbol Integrin alpha 1
Gene ID (NCBI) 3672
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

Protocols

Product Specific Protocols
WB protocol for Integrin alpha 1 antibody 68211-1-IgDownload protocol
IF protocol for Integrin alpha 1 antibody 68211-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols