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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Integrin alpha-5 Monoclonal antibody

Integrin alpha-5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B9E5

Cat no : 68561-1-Ig

Synonyms

CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin alpha-5 Monoclonal antibody (68561-1-Ig)

    WB analysis using 68561-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Integrin alpha-5 Monoclonal antibody (68561-1-Ig)

    WB analysis using 68561-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using Integrin alpha-5 Monoclonal antibody (68561-1-Ig)

    WB analysis of K-562 using 68561-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Integrin alpha-5 Monoclonal antibody (68561-1-Ig)

    WB analysis of Jurkat using 68561-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig colon tissue using Integrin alpha-5 Monoclonal antibody (68561-1-Ig)

    WB analysis of pig colon using 68561-1-Ig

    pig colon tissue were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, rat colon tissue, K-562 cells, Jurkat cells, pig colon tissue, HeLa cells, mouse colon tissue, mouse small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68561-1-Ig targets Integrin alpha-5 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Integrin alpha-5 fusion protein Ag33642 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Calculated molecular weight 115 kDa
Observed molecular weight140 kDa
GenBank accession numberBC008786
Gene symbol Integrin alpha 5
Gene ID (NCBI) 3678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

Protocols

Product Specific Protocols
WB protocol for Integrin alpha-5 antibody 68561-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols