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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Integrin alpha-5 Recombinant antibody, PBS Only (Capture)

Integrin alpha-5 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241685A3

Cat no : 84468-4-PBS

Synonyms

ITGA5, Integrin alpha-5 light chain, Integrin alpha-5 heavy chain, FNRA, CD49e

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin alpha-5 Recombinant antibody (84468-4-RR)

    WB analysis using 84468-4-RR (same clone as 84468-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84468-4-RR (Integrin alpha-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84468-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Integrin alpha-5 Recombinant antibody (84468-4-RR)

    IF Staining of HeLa using 84468-4-RR (same clone as 84468-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Integrin alpha-5 antibody (84468-4-RR, Clone: 241685A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84468-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01350-2

    Cytometric bead array standard curve of MP01350-2

    Cytometric bead array standard curve of MP01350-2, Integrin alpha-5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84468-4-PBS. Detection antibody: 84468-2-PBS. Standard: RP00012. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84468-4-RR

    Affinity and Kinetic Characterization of 84468-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84468-4-RR against Human Integrin alpha-5 were performed. The affinity constant is 1.24 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84468-4-PBS targets Integrin alpha-5 as part of a matched antibody pair:

MP01350-2: 84468-4-PBS capture and 84468-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Calculated molecular weight 115 kDa
Observed molecular weight140 kDa
GenBank accession numberBC008786
Gene symbol Integrin alpha 5
Gene ID (NCBI) 3678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).