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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin alpha-5 Recombinant antibody (84468-5-RR)

    WB analysis using 84468-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84468-5-RR (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84468-5-RR

    Affinity and Kinetic Characterization of 84468-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84468-5-RR against Human Integrin alpha-5 were performed. The affinity constant is 1.41 nM.

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Integrin alpha-5 Recombinant antibody

Integrin alpha-5 Recombinant Antibody for WB, ELISA
Cat No. 84468-5-RR
Clone No.241684E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ITGA5, Integrin alpha-5 light chain, Integrin alpha-5 heavy chain, FNRA, CD49e

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84468-5-RR targets Integrin alpha-5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Calculated molecular weight 115 kDa
Observed molecular weight140 kDa
GenBank accession numberBC008786
Gene Symbol Integrin alpha 5
Gene ID (NCBI) 3678
RRIDAB_3671992
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08648
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

Protocols

Product Specific Protocols
WB protocol for Integrin alpha-5 antibody 84468-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols