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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody

Integrin alpha-5/CD49e Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241685D4

Cat no : 98171-1-RR

Synonyms

Integrin Alpha-5, ITGA5, Integrin alpha-5 heavy chain, FNRA, CD49e

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human Integrin alpha-5/CD49e Rabbit Recombina (98171-1-RR)

    FC experiment of human PBMCs using 98171-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody (98171-1-RR, Clone: 241685D4) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human Integrin alpha-5/CD49e Rabbit Recombina (98171-1-RR)

    FC experiment of human PBMCs using 98171-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody (98171-1-RR, Clone: 241685D4) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98171-1-RR targets Integrin alpha-5/CD49e in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Calculated molecular weight 115 kDa
GenBank accession numberBC008786
Gene symbol Integrin alpha 5
Gene ID (NCBI) 3678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha-5 (ITGA5, also known as CD49e), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

Protocols

Product Specific Protocols
FC protocol for Integrin alpha-5/CD49e antibody 98171-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols