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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin alpha 6 Recombinant antibody (84463-3-RR)

    WB analysis using 84463-3-RR (same clone as 84463-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84463-3-RR (Integrin alpha 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84463-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Integrin alpha 6 Recombinant antibody (84463-3-RR)

    IF Staining of A431 using 84463-3-RR (same clone as 84463-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Integrin alpha-6 antibody (84463-3-RR, Clone: 241703B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 84463-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84463-3-RR

    Affinity and Kinetic Characterization of 84463-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84463-3-RR against Human Integrin alpha 6 were performed. The affinity constant is 0.614 nM.

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Integrin alpha 6 Recombinant antibody, PBS Only

Integrin alpha 6 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84463-3-PBS
Clone No.241703B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ITGA6, Integrin alpha-6 light chain, Integrin alpha-6 heavy chain, Integrin alpha-6, CD49f

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84463-3-PBS targets Integrin alpha 6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 6
Calculated molecular weight 1073 aa, 119 kDa
Observed molecular weight120 kDa
GenBank accession numberBC136456
Gene Symbol Integrin alpha 6
Gene ID (NCBI) 3655
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP23229
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.