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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin alpha-8 Polyclonal antibody (30714-1-AP)

    WB analysis using 30714-1-AP (same clone as 30714-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Integrin alpha-8 Polyclonal antibody (30714-1-AP)

    IHC staining of mouse lung using 30714-1-AP (same clone as 30714-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Integrin alpha-8 Polyclonal antibody (30714-1-AP)

    IHC staining of mouse lung using 30714-1-AP (same clone as 30714-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using Integrin alpha-8 Polyclonal antibody (30714-1-AP)

    IF Staining of mouse lung using 30714-1-AP (same clone as 30714-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Integrin alpha-8 antibody (30714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using Integrin alpha-8 Polyclonal antibody (30714-1-AP)

    IF Staining of mouse lung using 30714-1-AP (same clone as 30714-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Integrin alpha-8 antibody (30714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

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Integrin alpha-8 Polyclonal antibody, PBS Only

Integrin alpha-8 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 30714-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30714-1-PBS targets Integrin alpha-8 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin alpha-8 fusion protein Ag33507 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 8
Calculated molecular weight117 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_003638
Gene Symbol Integrin alpha 8
Gene ID (NCBI) 8516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53708
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. Integrin alpha-8 is a single-pass type I membrane protein that forms a heterodimer with integrin beta-1 to serve as a receptor for RGD-containing matrix molecules (fibronectin, vitronectin, tenascin C, osteopontin, and nephronectin) (PMID: 33000355). Integrin alpha-8 beta-1 plays a critical role in epithelial-mesenchymal interactions and functions in the genesis of kidney and probably of other organs (PMID: 9054500; 19769957). The apparent molecular weight of integrin alpha-8 is larger than the calculated molecular weight of 117 kDa, possibly due to glycosylation (PMID: 7768999).