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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin alpha-8 Polyclonal antibody (55068-1-AP)

    WB analysis using 55068-1-AP

    Mouse lung tissue was subjected to SDS PAGE followed by western blot with 55068-1-AP (Integrin alpha-8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Integrin alpha-8 Polyclonal antibody

Integrin alpha-8 Polyclonal Antibody for WB, ELISA
Cat No. 55068-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Integrin alpha 8, Integrin alpha-8, integrin, alpha 8, ITGA8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55068-1-AP targets Integrin alpha-8 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 8
Calculated molecular weight 117 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_003638
Gene Symbol Integrin alpha 8
Gene ID (NCBI) 8516
RRIDAB_3086432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53708
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. Integrin alpha-8 is a single-pass type I membrane protein that forms a heterodimer with integrin beta-1 to serve as a receptor for RGD-containing matrix molecules (fibronectin, vitronectin, tenascin C, osteopontin, and nephronectin) (PMID: 33000355). Integrin alpha-8 beta-1 plays a critical role in epithelial-mesenchymal interactions and functions in the genesis of kidney and probably of other organs (PMID: 9054500; 19769957). The apparent molecular weight of integrin alpha-8 is larger than the calculated molecular weight of 117 kDa, possibly due to glycosylation (PMID: 7768999).

Protocols

Product Specific Protocols
WB protocol for Integrin alpha-8 antibody 55068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols