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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Integrin Beta 4 Monoclonal antibody

Integrin Beta 4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C2A11

Cat no : 66922-2-Ig

Synonyms

ITGB4, integrin, beta 4, Integrin beta-4, GP150, CD104

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis using 66922-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SW 1990 cells using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis of SW 1990 using 66922-2-Ig

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis using 66922-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis of BxPC-3 using 66922-2-Ig

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis of A549 using 66922-2-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin beta-4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    IHC staining of human skin cancer using 66922-2-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    IHC staining of human skin cancer using 66922-2-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    IF Staining of A431 using 66922-2-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 4 antibody (66922-2-Ig, Clone: 1C2A11 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, BxPC-3 cells, SW 1990 cells, SKOV-3 cells, MCF-7 cells, Calu-3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66922-2-Ig targets Integrin Beta 4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Integrin Beta 4 fusion protein Ag17395 相同性解析による交差性が予測される生物種
Full Name integrin, beta 4
Calculated molecular weight 1752 aa, 195 kDa
Observed molecular weight 200-210 kDa
GenBank accession numberBC126411
Gene symbol Integrin beta 4
Gene ID (NCBI) 3691
RRIDAB_2882249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

Protocols

Product Specific Protocols
WB protocol for Integrin Beta 4 antibody 66922-2-IgDownload protocol
IHC protocol for Integrin Beta 4 antibody 66922-2-IgDownload protocol
IF protocol for Integrin Beta 4 antibody 66922-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols