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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin beta-5 Recombinant antibody (83514-3-RR)

    WB analysis using 83514-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Integrin beta-5 Recombinant antibody (83514-3-RR)

    IHC staining of human ovary cancer using 83514-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Integrin beta-5 Recombinant antibody (83514-3-RR)

    IHC staining of human ovary cancer using 83514-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83514-3-RR (Integrin beta-5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using Integrin beta-5 Recombinant antibody (83514-3-RR)

    FC experiment of HeLa using 83514-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Integrin beta-5 Recombinant antibody (83514-3-RR, Clone:240401G11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83514-3-RR

    Affinity and Kinetic Characterization of 83514-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83514-3-RR against Human Integrin beta-5 were performed. The affinity constant is 0.955 nM.

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Integrin beta-5 Recombinant antibody

Integrin beta-5 Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83514-3-RR
Clone No.240401G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

ITGB5, integrin, beta 5, Integrin beta 5, 240401G11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HT-29 cells, NIH-H1299 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83514-3-RR targets Integrin beta-5 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Integrin beta-5 fusion protein Ag29783 相同性解析による交差性が予測される生物種
Full Name integrin, beta 5
Calculated molecular weight 88 kDa
Observed molecular weight88-100 kDa
GenBank accession numberBC006541
Gene Symbol Integrin beta 5
Gene ID (NCBI) 3693
RRIDAB_3671139
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18084
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITGB5, also known as Integrin beta-5. It is expected to be located in the plasma membrane and mitochondria, which is i ubiquitinated in placenta and gall bladder. ITGB5 is a member of integrins and was proved to be associated with pathological processes in several tumors (PMID: 34966851). It is reported that TGB5 is highly expressed in hepatocellular carcinoma (HCC), and miR-185 regulates the expression of β- catenin through the ITGB5-dependent manner and affects the proliferation and migration of HCC cells (PMID: 35223869). The molecular weight of ITGB5 is 88 kDa, and the glycosylation modification also exists.

Protocols

Product Specific Protocols
WB protocol for Integrin beta-5 antibody 83514-3-RRDownload protocol
IHC protocol for Integrin beta-5 antibody 83514-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols