Involucrin Polyclonal antibody

Name: Involucrin Polyclonal antibody
Brand: Proteintech
SKU: 28462-1-AP

Involucrin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 28462-1-AP

Synonyms

IVL

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis of A431 using 28462-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 28462-1-AP (Involucrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 28462-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28462-1-AP (Involucrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 28462-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 28462-1-AP (Involucrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 28462-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 28462-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 28462-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 28462-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brown disease using 28462-1-AP
Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28462-1-AP (Involucrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 28462-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 28462-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 28462-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 28462-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 28462-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 28462-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 28462-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 28462-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using Involucrin antibody (28462-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of A431 using 28462-1-AP
1x10^6 A431 cells were intracellularly stained with 0.4 ug Involucrin Polyclonal antibody (28462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 28462-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HT-1080 cells, HT-29 cells
Positive IHC detected in	human tonsillitis tissue, human brown disease, human oesophagus cancer tissue, human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells
Positive FC (Intra) detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:200-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

28462-1-AP targets Involucrin in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Involucrin fusion protein Ag29585 相同性解析による交差性が予測される生物種
Full Name	involucrin
Calculated molecular weight	68 kDa
Observed molecular weight	120 kDa
GenBank accession number	NM_005547
Gene symbol	Involucrin
Gene ID (NCBI)	3713
RRID	AB_2881148
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502, 12150517)

Protocols

Product Specific Protocols
WB protocol for Involucrin antibody 28462-1-AP	Download protocol
IHC protocol for Involucrin antibody 28462-1-AP	Download protocol
IF protocol for Involucrin antibody 28462-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	J Nanobiotechnology Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration. Authors - Yunyao Bo View Article
	IF	Int Immunopharmacol Nicotinamide mononucleotide ameliorates DNFB-induced atopic dermatitis-like symptoms in mice by blocking activation of ROS-mediated JAK2/STAT5 signaling pathway. Authors - Jie-Fang Gao View Article
	WB,IHC	J Ethnopharmacol Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes. Authors - Zhenzhuo Li View Article
human	WB	J Oncol Downregulation of S100A9 Reverses Cisplatin-Resistance and Inhibits Proliferation and Migration in Hypopharyngeal Carcinoma Authors - Shiyu Zeng View Article
human	WB	Biol Pharm Bull Pyridoxine Has a Potential to Prevent the Appearance of Pigmented Spots: Effects on the Phagocytosis and Differentiation of Keratinocytes Authors - Yushi Katsuyama View Article
human	WB	Front Pharmacol Protective effect of Saussurea involucrata polysaccharide against skin dryness induced by ultraviolet radiation Authors - Lusheng Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Involucrin Polyclonal antibody

Involucrin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis of A431 using 28462-1-AP

WB analysis using 28462-1-AP

WB analysis of HT-1080 using 28462-1-AP

IHC staining of human tonsillitis using 28462-1-AP

IHC staining of human tonsillitis using 28462-1-AP

IHC staining of human skin cancer using 28462-1-AP

IHC staining of human skin cancer using 28462-1-AP

IHC staining of human brown disease using 28462-1-AP

IHC staining of human skin cancer using 28462-1-AP

IHC staining of human skin cancer using 28462-1-AP

IHC staining of human tonsillitis using 28462-1-AP

IHC staining of human oesophagus cancer using 28462-1-AP

IHC staining of human oesophagus cancer using 28462-1-AP

IHC staining of human oesophagus cancer using 28462-1-AP

IF Staining of A431 using 28462-1-AP

FC experiment of A431 using 28462-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.

Nicotinamide mononucleotide ameliorates DNFB-induced atopic dermatitis-like symptoms in mice by blocking activation of ROS-mediated JAK2/STAT5 signaling pathway.

Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.

Downregulation of S100A9 Reverses Cisplatin-Resistance and Inhibits Proliferation and Migration in Hypopharyngeal Carcinoma

Pyridoxine Has a Potential to Prevent the Appearance of Pigmented Spots: Effects on the Phagocytosis and Differentiation of Keratinocytes

Protective effect of Saussurea involucrata polysaccharide against skin dryness induced by ultraviolet radiation