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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JAK1 Monoclonal antibody (66466-1-Ig)

    WB analysis using 66466-1-Ig (same clone as 66466-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using JAK1 Monoclonal antibody (66466-1-Ig)

    WB analysis using 66466-1-Ig (same clone as 66466-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using JAK1 Monoclonal antibody (66466-1-Ig)

    WB analysis of rat spleen using 66466-1-Ig (same clone as 66466-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using JAK1 Monoclonal antibody (66466-1-Ig)

    IHC staining of human breast cancer using 66466-1-Ig (same clone as 66466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using JAK1 Monoclonal antibody (66466-1-Ig)

    IHC staining of human breast cancer using 66466-1-Ig (same clone as 66466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using JAK1 Monoclonal antibody (66466-1-Ig)

    IHC staining of human cervical cancer using 66466-1-Ig (same clone as 66466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using JAK1 Monoclonal antibody (66466-1-Ig)

    IHC staining of human cervical cancer using 66466-1-Ig (same clone as 66466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

JAK1 Monoclonal antibody, PBS Only

JAK1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66466-1-PBS
Clone No.3H7A8

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Synonyms

JAK 1, JAK1, JAK1A, JAK1B, Janus kinase 1, JTK3, Tyrosine protein kinase JAK1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66466-1-PBS targets JAK1 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen JAK1 fusion protein Ag17940 相同性解析による交差性が予測される生物種
Full Name Janus kinase 1 (a protein tyrosine kinase)
Calculated molecular weight 1154 aa, 133 kDa
Observed molecular weight 133 kDa
GenBank accession numberBC132729
Gene Symbol JAK1
Gene ID (NCBI) 3716
RRIDAB_2881834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23458
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.