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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JAK3 Recombinant antibody (80331-1-RR)

    WB analysis using 80331-1-RR (same clone as 80331-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80331-1-RR (JAK3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80331-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using JAK3 Recombinant antibody (80331-1-RR)

    IHC staining of human lymphoma using 80331-1-RR (same clone as 80331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 80331-1-RR (JAK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80331-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using JAK3 Recombinant antibody (80331-1-RR)

    IHC staining of human lymphoma using 80331-1-RR (same clone as 80331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 80331-1-RR (JAK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80331-1-PBS in a different storage buffer formulation.

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JAK3 Recombinant antibody, PBS Only

JAK3 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80331-1-PBS
Clone No.3N1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

3N1, EC:2.7.10.2, JAK 3, JAK-3, JAKL

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80331-1-PBS targets JAK3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Janus kinase 3 (a protein tyrosine kinase, leukocyte)
Calculated molecular weight 1124 aa, 125 kDa
Observed molecular weight 125 kDa
GenBank accession numberBC028068
Gene Symbol JAK3
Gene ID (NCBI) 3718
RRIDAB_2918888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52333
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

JAK3 is a non-receptor tyrosine kinase, predominantly expressed in hematopoietic cells and that has been implicated in the signal transduction of the common gamma chain subfamily of cytokine receptors. As a result, JAK3 plays an essential role in hematopoieisis during T cell development. JAK3 belongs to the Janus kinase family of receptor-associated tyrosine kinases located in cytoplasm adjacent to the plasma membrane. (PMID: 19747563, PMID: 33767339)