Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of mouse spleen using 13846-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 13846-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13846-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 13846-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13846-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13846-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 13846-1-AP
IP result of anti-JAKMIP1 (IP:13846-1-AP, 3ug; Detection:13846-1-AP 1:1000) with Jurkat cells lysate 4000ug.
IHC staining of human gliomas using 13846-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 13846-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

JAKMIP1 Polyclonal antibody

JAKMIP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13846-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

MARLIN1, Janus kinase and microtubule-interacting protein 1, janus kinase and microtubule interacting protein 1, JAMIP1, GABABRBP

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse spleen tissue, HEK-293 cells, Jurkat cells, mouse liver tissue, mouse testis tissue, Y79 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

13846-1-AP targets JAKMIP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	JAKMIP1 fusion protein Ag4816 相同性解析による交差性が予測される生物種
Full Name	janus kinase and microtubule interacting protein 1
Calculated molecular weight	73 kDa
Observed molecular weight	73 kDa
GenBank accession number	BC047075
Gene Symbol	JAKMIP1
Gene ID (NCBI)	152789
RRID	AB_2128793
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96N16
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for JAKMIP1 antibody 13846-1-AP	Download protocol
IHC protocol for JAKMIP1 antibody 13846-1-AP	Download protocol
IP protocol for JAKMIP1 antibody 13846-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Neuron JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse. Authors - Jamee M Berg KO Validated View Article
human	WB,IHC	Biomed Pharmacother Overexpression of JAKMIP1 associates with Wnt/beta-catenin pathway activation and promotes cancer cell proliferation in vitro. Authors - Okai Isaac I View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse spleen using 13846-1-AP

WB analysis of Y79 using 13846-1-AP

WB analysis of HEK-293 using 13846-1-AP

WB analysis of mouse liver using 13846-1-AP

WB analysis of mouse testis using 13846-1-AP

WB analysis of Jurkat using 13846-1-AP

IP experiment of Jurkat using 13846-1-AP

IHC staining of human gliomas using 13846-1-AP