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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using JAM-A/CD321 Recombinant antibody (84720-2-RR)

    WB analysis of HUVEC using 84720-2-RR

    HUVEC cells were subjected to SDS PAGE followed by western blot with 84720-2-RR (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood platelets using JAM-A/CD321 Recombinant antibody (84720-2-RR)

    WB analysis of human peripheral blood platelets using 84720-2-RR

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 84720-2-RR (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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JAM-A/CD321 Recombinant antibody

JAM-A/CD321 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84720-2-RR
Clone No.242290C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

F11R, JAM-A/Junctional Adhesion Molecule A, JAM 1, JAM, F11 receptor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHUVEC cells, human peripheral blood platelets

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84720-2-RR targets JAM-A/CD321 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name F11 receptor
Calculated molecular weight33kDa
Observed molecular weight38 kDa
GenBank accession numberNM_016946.6
Gene Symbol F11R
Gene ID (NCBI) 50848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y624-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and a ligand for the integrin LFA1.

Protocols

Product Specific Protocols
WB protocol for JAM-A/CD321 antibody 84720-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols