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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JMJD1C Polyclonal antibody (26637-1-AP)

    WB analysis using 26637-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26637-1-AP (JMJD1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using JMJD1C Polyclonal antibody (26637-1-AP)

    IF Staining of HeLa using 26637-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JMJD1C antibody (26637-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

JMJD1C Polyclonal antibody

JMJD1C Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26637-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

TRIP8, TRIP 8, JHDM2C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-251 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26637-1-AP targets JMJD1C in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JMJD1C fusion protein Ag24348 相同性解析による交差性が予測される生物種
Full Name jumonji domain containing 1C
Calculated molecular weight 2358 aa, 263 kDa
Observed molecular weight285 kDa
GenBank accession numberBC143722
Gene Symbol JMJD1C
Gene ID (NCBI) 221037
RRIDAB_3669557
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15652
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

JMJD1C, a member of the lysine demethylase 3 (KDM3) family, is universally required for the survival of several types of acute myeloid leukemia (AML) cells with different genetic mutations.

Protocols

Product Specific Protocols
WB protocol for JMJD1C antibody 26637-1-APDownload protocol
IF protocol for JMJD1C antibody 26637-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols