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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using JMJD4 Polyclonal antibody (21572-1-AP)

    WB analysis of HeLa using 21572-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21572-1-AP (JMJD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using JMJD4 Polyclonal antibody (21572-1-AP)

    WB analysis of HEK-293 using 21572-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21572-1-AP (JMJD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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JMJD4 Polyclonal antibody

JMJD4 Polyclonal Antibody for WB, ELISA
Cat No. 21572-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

JMJD4, jumonji domain containing 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21572-1-AP targets JMJD4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JMJD4 fusion protein Ag16099 相同性解析による交差性が予測される生物種
Full Name jumonji domain containing 4
Calculated molecular weight 463 aa, 52 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC109272
Gene Symbol JMJD4
Gene ID (NCBI) 65094
RRIDAB_2878886
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9V9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

JMJD4 (JmjC domain-containing protein 4, Jumonji domain-containing protein 4) is a member of the JmjC domain-containing proteins. Several JmjC domain-containing proteins have been reported to play a crucial role in the control of gene expression by acting as protein hydroxylases or histone demethylases (PMID: 16983801; 21300889). The function of JMJD4 protein remains unknown. The gene of JMJD4 maps to chromosome 1q42.13.

Protocols

Product Specific Protocols
WB protocol for JMJD4 antibody 21572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Hematol Oncol

JMJD4-demethylated RIG-I prevents hepatic steatosis and carcinogenesis

Authors - Zhenyang Li
View Article
humanWB

Circulation

Jmjd4 Facilitates Pkm2 Degradation in Cardiomyocytes and Is Protective Against Dilated Cardiomyopathy

Authors - Yansong Tang
  • KD Validated
View Article