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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JMJD5 Recombinant antibody (84804-4-RR)

    WB analysis using 84804-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84804-4-RR (JMJD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84804-4-RR

    Affinity and Kinetic Characterization of 84804-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84804-4-RR against Human JMJD5 were performed. The affinity constant is 43.3 pM.

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JMJD5 Recombinant antibody

JMJD5 Recombinant Antibody for WB, ELISA
Cat No. 84804-4-RR
Clone No.242097E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

JMJD 5, JmjC domain-containing protein 5, EC:1.14.11.73, Bifunctional peptidase and arginyl-hydroxylase JMJD5, 3R

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84804-4-RR targets JMJD5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen JMJD5 fusion protein Ag15115 相同性解析による交差性が予測される生物種
Full Name jumonji domain containing 5
Calculated molecular weight 416 aa, 47 kDa
Observed molecular weight50-60 kDa
GenBank accession numberBC027911
Gene Symbol JMJD5
Gene ID (NCBI) 79831
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8N371
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for JMJD5 antibody 84804-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols