JMY Polyclonal antibody
JMY Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 25098-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
25098-1-AP targets JMY in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | JMY fusion protein Ag18735 相同性解析による交差性が予測される生物種 |
| Full Name | junction mediating and regulatory protein, p53 cofactor |
| Calculated molecular weight | 988 aa, 111 kDa |
| Observed molecular weight | 111 kDa |
| GenBank accession number | BC130624 |
| Gene symbol | JMY |
| Gene ID (NCBI) | 133746 |
| RRID | AB_2879895 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Junction mediating and regulatory protein(JMY) is a 988 amino acid protein, which contains one WH2 domain and belongs to the JMY family. IIn nucleus, JMY acts both a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics in response to cellular stress. In cytoplasm, JMY acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Due to alternative splicing, several isoforms of JMY are produced, and these various isoforms have different influencing affects on p53 activation, with some isoforms markedly enhancing p53 responses compared to the other splicing variants.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for JMY antibody 25098-1-AP | Download protocol |
| IHC protocol for JMY antibody 25098-1-AP | Download protocol |
| IF protocol for JMY antibody 25098-1-AP | Download protocol |
| IP protocol for JMY antibody 25098-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Genet WHIMP links the actin nucleation machinery to Src-family kinase signaling during protrusion and motility. |
