JNK Polyclonal antibody

Name: JNK Polyclonal antibody
Brand: Proteintech
SKU: 10023-1-AP

JNK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10023-1-AP

Synonyms

MAPK8, JNK1A2, JNK1, JNK 2, JNK 1

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of NIH/3T3 using 10023-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10023-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 10023-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10023-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10023-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10023-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 10023-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10023-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10023-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10023-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10023-1-AP (JNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 10023-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10023-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 10023-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse heart using 10023-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 10023-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 10023-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10023-1-AP (JNK Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 10023-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JNK antibody (10023-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Cat No. 10023-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	NIH/3T3 cells, A431 cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, RAW 264.7 cells, RAW264.7, SH-SY5Y cells
Positive IHC detected in	human prostate cancer tissue, human lung cancer tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 29 publications below
IHC	See 3 publications below

Product Information

10023-1-AP targets JNK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	JNK fusion protein Ag0019 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase 8
Calculated molecular weight	48 kDa
Observed molecular weight	40-45 kDa, 50-55 kDa
GenBank accession number	NM_138982
Gene symbol	JNK
Gene ID (NCBI)	5599
RRID	AB_2281669
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.

Protocols

Product Specific Protocols
WB protocol for JNK antibody 10023-1-AP	Download protocol
IHC protocol for JNK antibody 10023-1-AP	Download protocol
IF protocol for JNK antibody 10023-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Pineal Res Melatonin reprogramming of gut microbiota improves lipid dysmetabolism in high fat diet-fed mice. Authors - Jie Yin View Article
human		Front Oncol Upregulation of OTUD7B (Cezanne) Promotes Tumor Progression via AKT/VEGF Pathway in Lung Squamous Carcinoma and Adenocarcinoma. Authors - Dan-Dan Lin View Article
human	WB	Neuropharmacology TRPC6-mediated ERK1/2 phosphorylation prevents dentate granule cell degeneration via inhibiting mitochondrial elongation. Authors - Ah-Reum Ko View Article
rat	WB	Ecotoxicol Environ Saf PM2.5 exposure induces reproductive injury through IRE1/JNK/autophagy signaling in male rats. Authors - Yang Yang View Article
mouse	WB	J Biol Chem Inactivation of mouse transmembrane prolyl 4-hydroxylase increases blood brain barrier permeability and ischemia-induced cerebral neuroinflammation. Authors - Nadiya Byts View Article
human	WB	Front Pharmacol BCATc inhibitor 2 ameliorated mitochondrial dysfunction and apoptosis in oleic acid-induced non-alcoholic fatty liver disease model Authors - Zhuo Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

JNK Polyclonal antibody

JNK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of NIH/3T3 using 10023-1-AP

WB analysis of MCF-7 using 10023-1-AP

WB analysis of RAW 264.7 using 10023-1-AP

WB analysis of MCF-7 using 10023-1-AP

WB analysis of HEK-293 using 10023-1-AP

WB analysis of SH-SY5Y using 10023-1-AP

WB analysis of RAW264.7 using 10023-1-AP

WB analysis of A431 using 10023-1-AP

WB analysis of HeLa using 10023-1-AP

WB analysis of Jurkat using 10023-1-AP

IHC staining of human prostate cancer using 10023-1-AP

IHC staining of human prostate cancer using 10023-1-AP

IHC staining of mouse heart using 10023-1-AP

IHC staining of mouse heart using 10023-1-AP

IHC staining of human lung cancer using 10023-1-AP

IHC staining of human lung cancer using 10023-1-AP

IF Staining of HeLa using 10023-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Function

Tissue specificity

Involvement in disease

Isoforms

Post-translational modifications

Cellular localization

Protocols

Publications

Melatonin reprogramming of gut microbiota improves lipid dysmetabolism in high fat diet-fed mice.

Upregulation of OTUD7B (Cezanne) Promotes Tumor Progression via AKT/VEGF Pathway in Lung Squamous Carcinoma and Adenocarcinoma.

TRPC6-mediated ERK1/2 phosphorylation prevents dentate granule cell degeneration via inhibiting mitochondrial elongation.

PM2.5 exposure induces reproductive injury through IRE1/JNK/autophagy signaling in male rats.

Inactivation of mouse transmembrane prolyl 4-hydroxylase increases blood brain barrier permeability and ischemia-induced cerebral neuroinflammation.

BCATc inhibitor 2 ameliorated mitochondrial dysfunction and apoptosis in oleic acid-induced non-alcoholic fatty liver disease model

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