JNK Polyclonal antibody
JNK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Conjugate
Unconjugated
158
Cat no : 24164-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, Jurkat cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Neuro-2a cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | A431 cells |
Positive IHC detected in | human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Jurkat cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 154 publications below |
IHC | See 5 publications below |
IF | See 2 publications below |
CoIP | See 1 publications below |
Product Information
24164-1-AP targets JNK in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | JNK fusion protein Ag21426 相同性解析による交差性が予測される生物種 |
Full Name | mitogen-activated protein kinase 8 |
Calculated molecular weight | 48 kDa |
Observed molecular weight | 42 kDa, 50 kDa |
GenBank accession number | BC130572 |
Gene symbol | JNK |
Gene ID (NCBI) | 5599 |
RRID | AB_2879443 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Function
JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.
Tissue specificity
JNK1 is ubiquitously expressed.
Involvement in disease
JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.
Isoforms
There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.
Post-translational modifications
JNK1 is phosphorylated by MAPK kinases (MAPKK).
Cellular localization
JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.
Protocols
Product Specific Protocols | |
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WB protocol for JNK antibody 24164-1-AP | Download protocol |
IHC protocol for JNK antibody 24164-1-AP | Download protocol |
IF protocol for JNK antibody 24164-1-AP | Download protocol |
IP protocol for JNK antibody 24164-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression | ||
Redox Biol FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response | ||
Transl Res Mitochondria transplantation protects traumatic brain injury via promoting neuronal survival and astrocytic BDNF. | ||
Br J Pharmacol Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A |