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  • Featured Product
  • KD/KO Validated

JNK Monoclonal antibody, PBS Only

JNK Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12E1

Cat no : 66210-1-PBS

Synonyms

MAPK8, JNK1, JNK 2, JNK 1, EC:2.7.11.24

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JNK Monoclonal antibody (66210-1-Ig)

    WB analysis using 66210-1-Ig (same clone as 66210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using JNK Monoclonal antibody (66210-1-Ig)

    WB analysis using 66210-1-Ig (same clone as 66210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using JNK Monoclonal antibody (66210-1-Ig)

    WB analysis of HeLa using 66210-1-Ig (same clone as 66210-1-PBS)

    Ethacrynic acid treated HeLa cells were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using JNK Monoclonal antibody (66210-1-Ig)

    WB analysis of RAW 264.7 using 66210-1-Ig (same clone as 66210-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using JNK Monoclonal antibody (66210-1-Ig)

    WB analysis of C6 using 66210-1-Ig (same clone as 66210-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using JNK Monoclonal antibody (66210-1-Ig)

    IHC staining of human lung cancer using 66210-1-Ig (same clone as 66210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using JNK Monoclonal antibody (66210-1-Ig)

    IHC staining of human lung cancer using 66210-1-Ig (same clone as 66210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using JNK Monoclonal antibody (66210-1-Ig)

    IHC staining of human prostate cancer using 66210-1-Ig (same clone as 66210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using JNK Monoclonal antibody (66210-1-Ig)

    IHC staining of human prostate cancer using 66210-1-Ig (same clone as 66210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using JNK Monoclonal antibody (66210-1-Ig)

    IF Staining of Jurkat using 66210-1-Ig (same clone as 66210-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (66210-1-Ig, Clone: 1A12E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using JNK Monoclonal antibody (66210-1-Ig)

    FC experiment of HeLa using 66210-1-Ig (same clone as 66210-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human JNK (66210-1-Ig, Clone:1A12E1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66210-1-PBS targets JNK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen JNK fusion protein Ag21426 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 8
Calculated molecular weight 48 kDa
Observed molecular weight 44-48 kDa, 50-55 kDa
GenBank accession numberBC130572
Gene symbol JNK
Gene ID (NCBI) 5599
RRIDAB_2881601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.