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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JNK Recombinant antibody (81629-1-RR)

    WB analysis using 81629-1-RR (same clone as 81629-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81629-1-RR (JNK antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using JNK Recombinant antibody (81629-1-RR)

    IHC staining of human colon cancer using 81629-1-RR (same clone as 81629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81629-1-RR (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using JNK Recombinant antibody (81629-1-RR)

    IHC staining of human colon cancer using 81629-1-RR (same clone as 81629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81629-1-RR (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using JNK Recombinant antibody (81629-1-RR)

    IF Staining of HeLa using 81629-1-RR (same clone as 81629-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JNK antibody (81629-1-RR, Clone: 6J16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using JNK Recombinant antibody (81629-1-RR)

    IF Staining of Jurkat using 81629-1-RR (same clone as 81629-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (81629-1-RR, Clone: 6J16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using JNK Recombinant antibody (81629-1-RR)

    FC experiment of HeLa using 81629-1-RR (same clone as 81629-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human JNK (81629-1-RR, Clone:6J16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81629-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81629-1-RR

    Affinity and Kinetic Characterization of 81629-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81629-1-RR against Human JNK were performed. The affinity constant is below 1 pM.

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JNK Recombinant antibody, PBS Only

JNK Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 81629-1-PBS
Clone No.6J16

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MAPK8, JNK1, JNK 2, JNK 1, EC:2.7.11.24

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81629-1-PBS targets JNK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen JNK fusion protein Ag1639 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 8
Calculated molecular weight 48 kDa
Observed molecular weight44-48 kDa, 50-55 kDa
GenBank accession numberBC130572
Gene Symbol JNK
Gene ID (NCBI) 5599
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP45983
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has some isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ). This antibody can recognize JNK1, JNK2 and JNK3.