JNK Recombinant antibody, PBS Only
JNK Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Host / Isotype
Rabbit / IgG
Reactivity
rat, mouse, human
Applications
WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Conjugate
Unconjugated
CloneNo.
6J16
Cat no : 81629-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
81629-1-PBS targets JNK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.
Tested Reactivity | rat, mouse, human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | JNK fusion protein Ag1639 相同性解析による交差性が予測される生物種 |
Full Name | mitogen-activated protein kinase 8 |
Calculated molecular weight | 48 kDa |
Observed molecular weight | 44-48 kDa, 50-55 kDa |
GenBank accession number | BC130572 |
Gene symbol | JNK |
Gene ID (NCBI) | 5599 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has some isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ). This antibody can recognize JNK1, JNK2 and JNK3.