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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JUN Monoclonal antibody (66313-1-Ig)

    WB analysis using 66313-1-Ig (same clone as 66313-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66313-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using JUN Monoclonal antibody (66313-1-Ig)

    WB analysis of ROS1728 using 66313-1-Ig (same clone as 66313-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66313-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using JUN Monoclonal antibody (66313-1-Ig)

    WB analysis of HEK-293 using 66313-1-Ig (same clone as 66313-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66313-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Saos-2 cells using JUN Monoclonal antibody (66313-1-Ig)

    WB analysis of Saos-2 using 66313-1-Ig (same clone as 66313-1-PBS)

    Saos-2 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66313-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using JUN Monoclonal antibody (66313-1-Ig)

    WB analysis of NIH/3T3 using 66313-1-Ig (same clone as 66313-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66313-1-PBS in a different storage buffer formulation.

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JUN Monoclonal antibody, PBS Only

JUN Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66313-1-PBS
Clone No.1G6B11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Activator protein 1, AP 1, AP1, c Jun, JUN, jun oncogene, P39, Proto oncogene c Jun, Transcription factor AP 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66313-1-PBS targets JUN in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen JUN fusion protein Ag17639 相同性解析による交差性が予測される生物種
Full Name jun oncogene
Calculated molecular weight 331 aa, 36 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC068522
Gene Symbol JUN
Gene ID (NCBI) 3725
RRIDAB_2881694
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05412
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

What is the molecular weight of JUN?

The molecular weight of JUN is approximately 39 kDa.

What is the cellular localization of JUN?

JUN expression can be located in the nucleus. However, ubiquitinated JUN colocalizes with lysosomal proteins (PMID: 15469925).

 What is the function of JUN?

JUN is a basic leucine zipper (bZIP) transcription factor that recognizes and binds to a consensus heptamer motif of 5'-TGA[CG]TCA-3' in the enhancer region of target genes. JUN acts as either a homo- or heterodimer and binds to the DNA to regulate transcriptional activity, specifically promoting the activity of NR5A1 when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon the stimulation of the cAMP signaling pathway (PMID: 9732876).

 What post-translational modifications is JUN subjected to?

JUN is acetylated at Lys-271 by EP300 (PMID: 11689449). JUN is phosphorylated by PLK3 following exposure to hypoxia or UV irradiation, which leads to increased DNA-binding activity (PMID: 27281822), and also by PAK2 at numerous threonine residues to promote cell proliferation and transformation (PMID: 21177766). JUN is also phosphorylated by DYRK2 at Ser-243, which primes it for subsequent further phosphorylation by GSK3B and reduces its ability to bind DNA (PMID: 22307329).

 What is the role of JUN in disease?

Activation of JUN is linked with proliferation and angiogenesis in invasive breast cancers (PMID: 16733206).