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KD/KO Validated

JUNB Polyclonal antibody

Name: JUNB Polyclonal antibody
Brand: Proteintech
SKU: 10486-1-AP

JUNB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10486-1-AP

Synonyms

AP-1, AP 1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of MCF-7 using 10486-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 10486-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 10486-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10486-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10486-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10486-1-AP
IP result of anti-JUNB (IP:10486-1-AP, 4ug; Detection:10486-1-AP 1:500) with HeLa cells lysate 1200ug.
IHC staining of human breast cancer using 10486-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10486-1-AP (JUNB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 10486-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10486-1-AP (JUNB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 10486-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10486-1-AP (JUNB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 10486-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 10486-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 10486-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 10486-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 10486-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUNB antibody (10486-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of A431 using 10486-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using JUNB antibody (10486-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Cat No. 10486-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF7 cells, C6 cells, HeLa cells, HT-1080 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lymphoma tissue, human breast cancer tissue, human ovary tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 8 publications below
IHC	See 1 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

10486-1-AP targets JUNB in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	JUNB fusion protein Ag0752 相同性解析による交差性が予測される生物種
Full Name	jun B proto-oncogene
Calculated molecular weight	36 kDa
Observed molecular weight	42 kDa, 43 kDa
GenBank accession number	BC004250
Gene symbol	JUNB
Gene ID (NCBI)	3726
RRID	AB_2129996
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context

Protocols

Product Specific Protocols
WB protocol for JUNB antibody 10486-1-AP	Download protocol
IHC protocol for JUNB antibody 10486-1-AP	Download protocol
IF protocol for JUNB antibody 10486-1-AP	Download protocol
IP protocol for JUNB antibody 10486-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration Authors - Jinpeng Du View Article
mouse	WB	J Exp Med USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein. Authors - Siyuan Chen View Article
mouse,rat	WB	Pharmacol Res Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways. Authors - Jiale Cai View Article
human	WB	Am J Cancer Res PMID: 31285951 Authors - Wen-Si Zhao View Article
human	WB	J Proteome Res Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells. Authors - Bing Wang View Article
human	WB	Exp Cell Res An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1 Authors - Xuanxuan Hu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

JUNB Polyclonal antibody

JUNB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of MCF-7 using 10486-1-AP

WB analysis of HT-1080 using 10486-1-AP

WB analysis of C6 using 10486-1-AP

WB analysis of MCF-7 using 10486-1-AP

WB analysis of HeLa using 10486-1-AP

IP experiment of HeLa using 10486-1-AP

IHC staining of human breast cancer using 10486-1-AP

IHC staining of human lymphoma using 10486-1-AP

IHC staining of human lymphoma using 10486-1-AP

IHC staining of human testis using 10486-1-AP

IHC staining of human testis using 10486-1-AP

IHC staining of human ovary using 10486-1-AP

IHC staining of human ovary using 10486-1-AP

IF Staining of HeLa using 10486-1-AP

IF Staining of A431 using 10486-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.

Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.

PMID: 31285951

Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells.

An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1