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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JUND Recombinant antibody (83134-6-RR)

    WB analysis using 83134-6-RR (same clone as 83134-6-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 83134-6-RR (JUND antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83134-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using JUND Recombinant antibody (83134-6-RR)

    IF Staining of HeLa using 83134-6-RR (same clone as 83134-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUND antibody (83134-6-RR, Clone: 230457A4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83134-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using JUND Recombinant antibody (83134-6-RR)

    FC experiment of A431 using 83134-6-RR (same clone as 83134-6-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug JUND Recombinant antibody (83134-6-RR, Clone:230457A4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83134-6-PBS in a different storage buffer formulation.

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JUND Recombinant antibody, PBS Only

JUND Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83134-6-PBS
Clone No.230457A4

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

AP 1, jun D proto oncogene, JUND, Transcription factor jun D

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83134-6-PBS targets JUND in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name jun D proto-oncogene
Calculated molecular weight 35 kDa
Observed molecular weight 35-45 kDa
GenBank accession numberNM_005354
Gene Symbol JUND
Gene ID (NCBI) 3727
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17535
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.