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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human Jagged1/CD339 Rabbit Recombinant Antibody

Jagged1/CD339 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241412A6

Cat no : 98243-1-RR

Synonyms

Jagged1, JAG1, Protein jagged-1, HJ1, CD339

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Human Jagged1/CD339 Rabbit Recombinant Antibo (98243-1-RR)

    FC experiment of Transfected HEK-293T using 98243-1-RR

    1x10^6 HEK-293T cells or Jagged1/CD339 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human Jagged1 Rabbit Recombinant Antibody (98243-1-RR, Clone:241412A6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Human Jagged1/CD339 Rabbit Recombinant Antibo (98243-1-RR)

    FC experiment of Transfected HEK-293T using 98243-1-RR

    1x10^6 HEK-293T cells (left) or Jagged1/CD339 transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Human Jagged1/CD339 Rabbit Recombinant Antibody (98243-1-RR, Clone:241412A6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98243-1-RR

    Affinity and Kinetic Characterization of 98243-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98243-1-RR against Human Jagged1/CD339 were performed. The affinity constant is 1.57 nM.

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Tested Applications

Positive FC detected inTransfected HEK-293T cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98243-1-RR targets Jagged1/CD339 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name jagged 1 (Alagille syndrome)
Calculated molecular weight 1218 aa, 134 kDa
GenBank accession numberBC098393
Gene symbol Jagged1
Gene ID (NCBI) 182
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Jagged1 (JAG1), also known as CD339, is a type I, membrane-anchored, multi-domain protein and a ligand of the Notch receptor (PMID: 15358557). Jagged1 is composed of a relatively small intracellular domain, a transmembrane domain, and a larger extracellular component containing a cystine-rich region, 16 EGF-like repeats, a DSL domain, and a signal peptide (PMID: 15358557; 26548814). It is widely expressed throughout mammalian development, across many tissues and developmental stages (PMID: 26548814). The Jagged1-NOTCH interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis.

Protocols

Product Specific Protocols
FC protocol for Jagged1/CD339 antibody 98243-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols