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- KD/KO Validated
KALRN Polyclonal antibody
KALRN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 19740-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse colon tissue, human kidney tissue, human brain tissue, rat colon tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
19740-1-AP targets KALRN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | kalirin, RhoGEF kinase |
| Calculated molecular weight | 340 kDa |
| Observed molecular weight | 200 kDa |
| GenBank accession number | NM_007064 |
| Gene symbol | KALRN |
| Gene ID (NCBI) | 8997 |
| RRID | AB_10638319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KALRN, also named as DUET, DUO, HAPIP and TRAD, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. KALRN promotes the exchange of GDP by GTP. KALRN activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. KALRN induces lamellipodia independent of its GEF activity. The antibody can recognize isoform1 and isoform2 of KALRN.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KALRN antibody 19740-1-AP | Download protocol |
| IHC protocol for KALRN antibody 19740-1-AP | Download protocol |
| IF protocol for KALRN antibody 19740-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FEBS Lett Kalirin mediates Rac1 activation downstream of calcium/calmodulin-dependent protein kinase II to stimulate glucose uptake during muscle contraction. | ||
Biochem Biophys Res Commun CaMKII and Kalirin, a Rac1-GEF, regulate Akt phosphorylation involved in contraction-induced glucose uptake in skeletal muscle cells. | ||
BMC Womens Health Metabolism-associated molecular classification of cervical cancer
| ||
Mol Cell Neurosci Role and mechanism of EphB3 in epileptic seizures and epileptogenesis through Kalirin |
