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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KANK1 Polyclonal antibody (29782-1-AP)

    WB analysis using 29782-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29782-1-AP (KANK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using KANK1 Polyclonal antibody (29782-1-AP)

    IHC staining of mouse heart using 29782-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29782-1-AP (KANK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using KANK1 Polyclonal antibody (29782-1-AP)

    IHC staining of mouse heart using 29782-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29782-1-AP (KANK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using KANK1 Polyclonal antibody (29782-1-AP)

    IF Staining of mouse kidney using 29782-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using KANK1 antibody (29782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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KANK1 Polyclonal antibody

KANK1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 29782-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

KN motif and ankyrin repeat domain-containing protein 1, Kidney ankyrin repeat-containing protein, KIAA0172, KANK, Ankyrin repeat domain-containing protein 15

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29782-1-AP targets KANK1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KANK1 fusion protein Ag31422 相同性解析による交差性が予測される生物種
Full Name KN motif and ankyrin repeat domains 1
Calculated molecular weight147KD
Observed molecular weight130-200 kDa
GenBank accession numberNM_015158
Gene Symbol KANK1
Gene ID (NCBI) 23189
RRIDAB_3086160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14678
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KANK1 (KN motif and ankyrin repeat domain-containing protein 1) is involved in the control of cytoskeleton formation by regulating actin polymerization, inhibiting actin fiber formation and cell migration (PMID: 25961457). It inhibits RhoA activity, the formation of lamellipodia but not of filopodia (PMID: 25961457, PMID: 5961457).It also inhibits fibronectin-mediated cell spreading and regulates Rac signaling pathways (PMID: 25961457). Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions (PMID: 27410476). KANK1 can be detected 130-200 kDa (PMID: 15823577, PMID: 33253712, PMID: 35805114).

Protocols

Product Specific Protocols
WB protocol for KANK1 antibody 29782-1-APDownload protocol
IHC protocol for KANK1 antibody 29782-1-APDownload protocol
IF protocol for KANK1 antibody 29782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols