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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KANK1 Monoclonal antibody (68613-1-Ig)

    WB analysis using 68613-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68613-1-Ig (KANK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KANK1 Monoclonal antibody (68613-1-Ig)

    IF Staining of HepG2 using 68613-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KANK1 antibody (68613-1-Ig, Clone: 2D3G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of HepG2 cells using KANK1 Monoclonal antibody (68613-1-Ig)

    FC experiment of HepG2 using 68613-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KANK1 Monoclonal antibody (68613-1-Ig, Clone:2D3G8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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KANK1 Monoclonal antibody

KANK1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 68613-1-Ig
Clone No.2D3G8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

KN motif and ankyrin repeat domain-containing protein 1, Kidney ankyrin repeat-containing protein, KIAA0172, KANK, Ankyrin repeat domain-containing protein 15

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, PC-3 cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, HuH-7 cells, Daudi cells, Raji cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68613-1-Ig targets KANK1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KANK1 fusion protein Ag31440 相同性解析による交差性が予測される生物種
Full Name KN motif and ankyrin repeat domains 1
Calculated molecular weight147KD
Observed molecular weight130-200 kDa
GenBank accession numberNM_015158
Gene Symbol KANK1
Gene ID (NCBI) 23189
RRIDAB_3085307
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14678
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KANK1 (KN motif and ankyrin repeat domain-containing protein 1) is involved in the control of cytoskeleton formation by regulating actin polymerization, inhibiting actin fiber formation and cell migration (PMID: 25961457). It inhibits RhoA activity, the formation of lamellipodia but not of filopodia (PMID: 25961457, PMID: 5961457).It also inhibits fibronectin-mediated cell spreading and regulates Rac signaling pathways (PMID: 25961457). Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions (PMID: 27410476). KANK1 can be detected 130-200 kDa (PMID: 15823577, PMID: 33253712, PMID: 35805114).

Protocols

Product Specific Protocols
WB protocol for KANK1 antibody 68613-1-IgDownload protocol
IF protocol for KANK1 antibody 68613-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols