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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

KANK2 Polyclonal antibody

KANK2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20546-1-AP

Synonyms

MXRA3, Matrix-remodeling-associated protein 3, KN motif and ankyrin repeat domain-containing protein 2, KIAA1518, Ankyrin repeat domain-containing protein 25

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using KANK2 Polyclonal antibody (20546-1-AP)

    IHC staining of human colon using 20546-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20546-1-AP (KANK2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using KANK2 Polyclonal antibody (20546-1-AP)

    IHC staining of human colon using 20546-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20546-1-AP (KANK2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using KANK2 Polyclonal antibody (20546-1-AP)

    IHC staining of mouse ovary using 20546-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 20546-1-AP (KANK2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using KANK2 Polyclonal antibody (20546-1-AP)

    IHC staining of mouse heart using 20546-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20546-1-AP (KANK2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using KANK2 Polyclonal antibody (20546-1-AP)

    IHC staining of human kidney using 20546-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20546-1-AP (KANK2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using KANK2 Polyclonal antibody (20546-1-AP)

    IHC staining of human kidney using 20546-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20546-1-AP (KANK2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KANK2 Polyclonal antibody (20546-1-AP)

    IF Staining of HeLa using 20546-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KANK2 antibody (20546-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive IHC detected inhuman colon tissue, human kidney tissue, mouse heart tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

20546-1-AP targets KANK2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name KN motif and ankyrin repeat domains 2
Calculated molecular weight 91 kDa
GenBank accession numberNM_001136191
Gene symbol KANK2
Gene ID (NCBI) 25959
RRIDAB_10694286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KANK2, also named as ANKRD25, KIAA1518, MXRA3 and SIP, is found as a candidate tumor suppressor for renal cell carcinoma. It is an ankyrin-repeat domain-containing protein. KANK2 has an identical role in the formation of actin stress fibers. The antibody is specific to KANK2.

Protocols

Product Specific Protocols
IHC protocol for KANK2 antibody 20546-1-APDownload protocol
IF protocol for KANK2 antibody 20546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Lab Med

Identification of biomarkers associated with pathological tumor staging and their utility in the diagnosis and prognosis of prostate cancer

Authors - Shiquan Xu
View Article