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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KAP1 Monoclonal antibody (66630-1-Ig)

    WB analysis of HeLa using 66630-1-Ig (same clone as 66630-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66630-1-Ig (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using KAP1 Monoclonal antibody (66630-1-Ig)

    WB analysis of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

    WB result of KAP1 antibody (66630-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAP1 transfected HepG2 cells. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KAP1 Monoclonal antibody (66630-1-Ig)

    WB analysis using 66630-1-Ig (same clone as 66630-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66630-1-Ig (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using KAP1 Monoclonal antibody (66630-1-Ig)

    IP experiment of HeLa using 66630-1-Ig (same clone as 66630-1-PBS)

    IP result of anti-KAP1 (IP:66630-1-Ig, 5ug; Detection:66630-1-Ig 1:1000) with HeLa cells lysate 4000 ug. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KAP1 Monoclonal antibody (66630-1-Ig)

    IHC staining of human breast cancer using 66630-1-Ig (same clone as 66630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KAP1 Monoclonal antibody (66630-1-Ig)

    IHC staining of human colon cancer using 66630-1-Ig (same clone as 66630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KAP1 Monoclonal antibody (66630-1-Ig)

    IHC staining of human colon cancer using 66630-1-Ig (same clone as 66630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KAP1 Monoclonal antibody (66630-1-Ig)

    IHC staining of human breast cancer using 66630-1-Ig (same clone as 66630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KAP1 Monoclonal antibody (66630-1-Ig)

    IF Staining of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (66630-1-Ig, Clone: 1B9G12 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KAP1 Monoclonal antibody (66630-1-Ig)

    IF Staining of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (66630-1-Ig, Clone: 1B9G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KAP1 Monoclonal antibody, PBS Only

KAP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66630-1-PBS
Clone No.1B9G12

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

TRIM28, KRAB associated protein 1, KAP-1, KAP 1, EC:2.3.2.27

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66630-1-PBS targets KAP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen KAP1 fusion protein Ag7519 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 28
Calculated molecular weight 89 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC004978
Gene Symbol KAP1
Gene ID (NCBI) 10155
RRIDAB_2732886
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13263
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )