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KARS Polyclonal antibody
KARS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 14951-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, Jurkat cells, human colon tissue, HeLa cells, HepG2 cells |
| Positive IHC detected in | human brown disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IF | See 3 publications below |
Product Information
14951-1-AP targets KARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KARS fusion protein Ag6778 相同性解析による交差性が予測される生物種 |
| Full Name | lysyl-tRNA synthetase |
| Calculated molecular weight | 68 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC004132 |
| Gene symbol | KARS |
| Gene ID (NCBI) | 3735 |
| RRID | AB_2128158 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KARS (lysyl-tRNA synthetase) is a gene that encodes an enzyme critical for protein synthesis, specifically responsible for attaching the amino acid lysine to its corresponding transfer RNA (tRNA) in both the cytoplasm and mitochondria. This enzyme plays a crucial role in the process of aminoacylation, which is essential for accurate protein translation from mRNA sequences. Mutations in the KARS gene have been associated with a variety of neurological and systemic disorders, reflecting the enzyme's importance in cellular function.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KARS antibody 14951-1-AP | Download protocol |
| IHC protocol for KARS antibody 14951-1-AP | Download protocol |
| IF protocol for KARS antibody 14951-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Lysyl-tRNA synthetase produces diadenosine tetraphosphate to curb STING-dependent inflammation. | ||
Nucleic Acids Res Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function. | ||
Nucleic Acids Res Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex. | ||
Cell Chem Biol Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer. | ||
Hum Mutat Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis. | ||
Front Cell Infect Microbiol Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kishor (Verified Customer) (01-30-2019) | This antibody working well against rat liver tissue and human cancer cells
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