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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using KAT2A/GCN5 Polyclonal antibody (28390-1-AP)

    WB analysis of HUVEC using 28390-1-AP (same clone as 28390-1-PBS)

    WB result of KAT2A/GCN5 antibody (28390-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and KAT2A/GCN5 knockout HUVEC cells. This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KAT2A/GCN5 Polyclonal antibody (28390-1-AP)

    WB analysis using 28390-1-AP (same clone as 28390-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28390-1-AP (KAT2A/GCN5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of hTERT-RPE1 cells using KAT2A/GCN5 Polyclonal antibody (28390-1-AP)

    IP experiment of hTERT-RPE1 using 28390-1-AP (same clone as 28390-1-PBS)

    IP result of anti-KAT2A (IP:28390-1-AP, 4ug; Detection:28390-1-AP 1:400) with hTERT-RPE1 cells lysate 1645 ug. This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of LNCaP cells using KAT2A/GCN5 Polyclonal antibody (28390-1-AP)

    IP experiment of LNCaP using 28390-1-AP (same clone as 28390-1-PBS)

    IP result of anti-KAT2A/GCN5 (IP:28390-1-AP, 4ug; Detection:28390-1-AP 1:6000) with LNCaP cells lysate 1200 ug. This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KAT2A/GCN5 Polyclonal antibody (28390-1-AP)

    IF Staining of HeLa using 28390-1-AP (same clone as 28390-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KAT2A/GCN5 antibody (28390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28390-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KAT2A/GCN5 Polyclonal antibody, PBS Only

KAT2A/GCN5 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 28390-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

hGCN5, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28390-1-PBS targets KAT2A/GCN5 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KAT2A/GCN5 fusion protein Ag29005 相同性解析による交差性が予測される生物種
Full Name K(lysine) acetyltransferase 2A
Calculated molecular weight 94 kDa
Observed molecular weight94 kDa
GenBank accession numberBC032743
Gene Symbol KAT2A
Gene ID (NCBI) 2648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92830
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.