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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCAF Polyclonal antibody

PCAF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 28770-1-AP

Synonyms

CAF, Histone acetylase PCAF, Histone acetyltransferase PCAF, K(lysine) acetyltransferase 2B, KAT2B, Lysine acetyltransferase 2B, P/CAF, P300/CBP associated factor, PCAF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCAF Polyclonal antibody (28770-1-AP)

    WB analysis using 28770-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28770-1-AP (KAT2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA431 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28770-1-AP targets PCAF in WB, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCAF fusion protein Ag30238 相同性解析による交差性が予測される生物種
Full Name K(lysine) acetyltransferase 2B
Calculated molecular weight 93 kDa
Observed molecular weight95-110 kDa
GenBank accession numberBC060823
Gene symbol KAT2B
Gene ID (NCBI) 8850
RRIDAB_2918200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCAF antibody 28770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

J Exp Clin Cancer Res

The histone lysine acetyltransferase KAT2B inhibits cholangiocarcinoma growth: evidence for interaction with SP1 to regulate NF2-YAP signaling

Authors - Wenbo Ma
View Article
humanWB

J Cell Mol Med

SIRT2-mediated deacetylation of ACLY promotes the progression of oesophageal squamous cell carcinoma

Authors - Xueying Zhang
View Article