- Featured Product
- KD/KO Validated
TIP60/KAT5 Polyclonal antibody
TIP60/KAT5 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 10827-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, rat liver tissue, HepG2 cells, Jurkat cells, HeLa cells, mouse liver tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 18 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
10827-1-AP targets TIP60/KAT5 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TIP60/KAT5 fusion protein Ag1261 相同性解析による交差性が予測される生物種 |
| Full Name | K(lysine) acetyltransferase 5 |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 58 kDa, 70 kDa |
| GenBank accession number | BC000166 |
| Gene symbol | KAT5 |
| Gene ID (NCBI) | 10524 |
| RRID | AB_2128431 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KAT5, also named as The histone acetyltransferase (HAT) Tat interactive protein 60 kD belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis. KAT5 exists some isoforms with MV 53-60 kDa
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TIP60/KAT5 antibody 10827-1-AP | Download protocol |
| IHC protocol for TIP60/KAT5 antibody 10827-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Immunol The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells. | ||
Nat Commun Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress.
| ||
Cancer Lett Acetylation-dependent deubiquitinase USP26 stabilizes BAG3 to promote breast cancer progression | ||
J Mol Cell Biol Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis |
