Validation Data Gallery
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Tested Applications
| Positive WB detected in | HCT 116 cells, mouse brain tissue, rat brain tissue, HeLa cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human bowen disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 11 publications below |
| IF | See 7 publications below |
Product Information
17560-1-AP targets KATNA1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11753 Product name: Recombinant human KATNA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-311 aa of BC050428 Sequence: MSLLMISENVKLAREYALLGNYDSAMVYYQGVLDQMNKYLYSVKDTYLQQKWQQVWQEINVEAKHVKDIMKTLESFKLDSTPLKAAQHDLPASEGEVWSMPVPVERRPSPGPRKRQSSQYSDPKSHGNRPSTTVRVHRSSAQNVHNDRGKAVRCREKKEQNKGREEKGVLMVGPPGTGKTLLAKAVATECKTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYSPATIFIDEIDSICSRRGTSEEHEASRRVKAELLVQMDGVGGTSENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPSGMRP 相同性解析による交差性が予測される生物種 |
| Full Name | katanin p60 (ATPase-containing) subunit A 1 |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC050428 |
| Gene Symbol | KATNA1 |
| Gene ID (NCBI) | 11104 |
| RRID | AB_10694670 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75449 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KATNA1 antibody 17560-1-AP | Download protocol |
| IP protocol for KATNA1 antibody 17560-1-AP | Download protocol |
| WB protocol for KATNA1 antibody 17560-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly. | ||
Nat Cell Biol Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex. | ||
Neuron Katanin p80 regulates human cortical development by limiting centriole and cilia number. | ||
PLoS Biol Primary cilium loss in mammalian cells occurs predominantly by whole-cilium shedding. | ||
Elife Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission |
