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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using KBTBD2 Polyclonal antibody (13871-1-AP)

    WB analysis of COLO 320 using 13871-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13871-1-AP (KBTBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KBTBD2 Polyclonal antibody (13871-1-AP)

    WB analysis using 13871-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13871-1-AP (KBTBD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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KBTBD2 Polyclonal antibody

KBTBD2 Polyclonal Antibody for WB, ELISA
Cat No. 13871-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

BKLHD1, KBTBD2, KIAA1489

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13871-1-AP targets KBTBD2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KBTBD2 fusion protein Ag4823 相同性解析による交差性が予測される生物種
Full Name kelch repeat and BTB (POZ) domain containing 2
Calculated molecular weight 622 aa, 71 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC047107
Gene Symbol KBTBD2
Gene ID (NCBI) 25948
RRIDAB_2296330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IY47
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106). A 60-70 kDa band has also been reported(PMID:27708159).

Protocols

Product Specific Protocols
WB protocol for KBTBD2 antibody 13871-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols