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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KBTBD5 Polyclonal antibody

KBTBD5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21836-1-AP

Synonyms

KBTBD5, KLHL40, Sarcosynapsin, SRYP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using KBTBD5 Polyclonal antibody (21836-1-AP)

    WB analysis of mouse skeletal muscle using 21836-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21836-1-AP (KBTBD5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using KBTBD5 Polyclonal antibody (21836-1-AP)

    IHC staining of mouse skeletal muscle using 21836-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21836-1-AP (KBTBD5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using KBTBD5 Polyclonal antibody (21836-1-AP)

    IHC staining of mouse skeletal muscle using 21836-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21836-1-AP (KBTBD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using KBTBD5 Polyclonal antibody (21836-1-AP)

    IHC staining of human skeletal muscle using 21836-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21836-1-AP (KBTBD5 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using KBTBD5 Polyclonal antibody (21836-1-AP)

    IHC staining of human skeletal muscle using 21836-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21836-1-AP (KBTBD5 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21836-1-AP targets KBTBD5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KBTBD5 fusion protein Ag16430 相同性解析による交差性が予測される生物種
Full Name kelch repeat and BTB (POZ) domain containing 5
Calculated molecular weight 621 aa, 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC110491
Gene symbol KBTBD5
Gene ID (NCBI) 131377
RRIDAB_2878923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KBTBD5, also known as KLHL40 and SRYP, contains a BACK domain, a BTB/POZ domain and 5 Kelch repeats. It is expressed predominantly in skeletal muscle. The KLHL40 gene mutation is responsible for a severe and potentially fatal neuromyopathy (PMID: 38397198).

Protocols

Product Specific Protocols
WB protocol for KBTBD5 antibody 21836-1-APDownload protocol
IHC protocol for KBTBD5 antibody 21836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols