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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KBTBD8 Polyclonal antibody (31970-1-AP)

    WB analysis using 31970-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31970-1-AP (KBTBD8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  • Featured Product
  • KD/KO Validated

KBTBD8 Polyclonal antibody

KBTBD8 Polyclonal Antibody for WB, ELISA
Cat No. 31970-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

T-cell activation kelch repeat protein, TA-KRP, TAKRP, KIAA1842, Kelch repeat and BTB domain-containing protein 8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDaudi cells, RKO cells, U-118 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31970-1-AP targets KBTBD8 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KBTBD8 fusion protein Ag36219 相同性解析による交差性が予測される生物種
Full Name kelch repeat and BTB (POZ) domain containing 8
Calculated molecular weight69 kDa，601aa
Observed molecular weight60 kDa
GenBank accession numberNM_032505.2
Gene Symbol KBTBD8
Gene ID (NCBI) 84541
RRIDAB_3670161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8NFY9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kelch repeat and BTB domain containing 8 (KBTBD8, also known as TAKRP and TA-KRP) is a member of the BTB-kelch family of proteins, which is specifically localized at the Golgi apparatus in nondividing cells and becomes restricted to the spindle apparatus upon mitosis (PMID: 23578279). KBTBD family proteins are involved in ubiquitination in various cells and tissues and are correlated with human muscle diseases (PMID: 9878064; 16547521; 24959344). The expression level of KBTBD8 might affect the proliferation and migration of epithelial ovarian cancer (PMID: 33109073).

Protocols

Product Specific Protocols
WB protocol for KBTBD8 antibody 31970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols