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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using KCNE1 Polyclonal antibody (15150-1-AP)

    WB analysis of mouse heart using 15150-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15150-1-AP (KCNE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using KCNE1 Polyclonal antibody (15150-1-AP)

    WB analysis of mouse testis using 15150-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15150-1-AP (KCNE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using KCNE1 Polyclonal antibody (15150-1-AP)

    IF Staining of mouse heart tissue using 15150-1-AP

    IF result of anti-KCNE1 (15150-1-AP) in frozen mouse heart section by Dr Nicola Smart.

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KCNE1 Polyclonal antibody

KCNE1 Polyclonal Antibody for WB, IF, ELISA
Cat No. 15150-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Synonyms

ISK, JLNS, JLNS2, KCNE1, LQT2/5, LQT5, Minimal potassium channel, MinK

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, mouse testis tissue
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15150-1-AP targets KCNE1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNE1 fusion protein Ag3495 相同性解析による交差性が予測される生物種
Full Name potassium voltage-gated channel, Isk-related family, member 1
Calculated molecular weight 15 kDa
Observed molecular weight 18-25 kDa, 28-35 kDa
GenBank accession numberBC036452
Gene Symbol KCNE1
Gene ID (NCBI) 3753
RRIDAB_2131977
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15382
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KCNE1 antibody 15150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Br J Pharmacol

Aldosterone downregulates slowly activated delayed rectifier potassium current in adult guinea pig cardiomyocytes.

Authors - Yankun Lv
View Article
ratWB

Cardiovasc Ther

LCZ696 Therapy Reduces Ventricular Tachyarrhythmia Inducibility in a Myocardial Infarction-Induced Heart Failure Rat Model.

Authors - Po-Cheng Chang
View Article