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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCNE2 Polyclonal antibody (17920-1-AP)

    WB analysis using 17920-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17920-1-AP (KCNE2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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KCNE2 Polyclonal antibody

KCNE2 Polyclonal Antibody for WB, ELISA
Cat No. 17920-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

MinK-related peptide 1, MinK related peptide 1, Minimum potassium ion channel-related peptide 1, LQT6, LQT5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue, mouse pancreas tissue, rat kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17920-1-AP targets KCNE2 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNE2 fusion protein Ag12336 相同性解析による交差性が予測される生物種
Full Name potassium voltage-gated channel, Isk-related family, member 2
Calculated molecular weight 123 aa, 14 kDa
Observed molecular weight30 kDa
GenBank accession numberBC093892
Gene Symbol KCNE2
Gene ID (NCBI) 9992
RRIDAB_3669283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6J6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Potassium voltage-gated channel subfamily E regulatory subunit 2 (KCNE2, also known as LQT5, LQT6, ATFB4, and MIRP1) functions as a regulatory subunit of the voltage-gated potassium (Kv) channel complex composed of pore-forming and potassium-conducting alpha subunits and of regulatory beta subunits (PMID: 10219239; 11034315; 11101505; 12185453; 20533308). KCNE2 beta subunit modulates the gating kinetics and enhances the stability of the channel complex (PMID: 10219239; 11034315; 11101505; 12185453; 20533308). It is expressed in various parts of the heart, with higher levels observed in the ventricles and Purkinje tissues compared to the atria (PMID: 39272981). KCNE2 has three forms, such as the core (15 kDa), singly glycosylated (20 kDa), and doubly glycosylated (25 kDa) forms (PMID: 15066947).

Protocols

Product Specific Protocols
WB protocol for KCNE2 antibody 17920-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols