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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KChIP1 Polyclonal antibody

KChIP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14212-1-AP

Synonyms

KCHIP1, KCNIP1, VABP, Vesicle APC binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human cerebellum tissue using KChIP1 Polyclonal antibody (14212-1-AP)

    WB analysis of human cerebellum using 14212-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14212-1-AP (KChIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using KChIP1 Polyclonal antibody (14212-1-AP)

    WB analysis of human brain using 14212-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14212-1-AP (KChIP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of fetal human brain tissue using KChIP1 Polyclonal antibody (14212-1-AP)

    IP experiment of fetal human brain using 14212-1-AP

    IP result of anti-KChIP1 (IP:14212-1-AP, 4ug; Detection:14212-1-AP 1:300) with fetal human brain tissue lysate 3500 ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KChIP1 Polyclonal antibody (14212-1-AP)

    IHC staining of human pancreas cancer using 14212-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14212-1-AP (KChIP1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KChIP1 Polyclonal antibody (14212-1-AP)

    IHC staining of human pancreas cancer using 14212-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14212-1-AP (KChIP1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inhuman cerebellum tissue, human brain tissue
Positive IP detected infetal human brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14212-1-AP targets KChIP1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KChIP1 fusion protein Ag5432 相同性解析による交差性が予測される生物種
Full Name Kv channel interacting protein 1
Calculated molecular weight 227 aa, 27 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC050375
Gene symbol KCNIP1
Gene ID (NCBI) 30820
RRIDAB_2265412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.

Protocols

Product Specific Protocols
WB protocol for KChIP1 antibody 14212-1-APDownload protocol
IHC protocol for KChIP1 antibody 14212-1-APDownload protocol
IP protocol for KChIP1 antibody 14212-1-APDownload protocol
FC protocol for KChIP1 antibody 14212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols