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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Kir2.2 Polyclonal antibody

Kir2.2 Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 14135-1-AP

Synonyms

KCNJ12, IRK2, IRK 2, hkir2.2x, hIRK1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using Kir2.2 Polyclonal antibody (14135-1-AP)

    WB analysis of mouse lung using 14135-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14135-1-AP (Kir2.2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lung tissue using Kir2.2 Polyclonal antibody (14135-1-AP)

    WB analysis of rat lung using 14135-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 14135-1-AP (Kir2.2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HEK-293 cells using Kir2.2 Polyclonal antibody (14135-1-AP)

    FC experiment of HEK-293 using 14135-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug Kir2.2 Polyclonal antibody (14135-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse lung tissue, rat lung tissue
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14135-1-AP targets Kir2.2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kir2.2 fusion protein Ag5349 相同性解析による交差性が予測される生物種
Full Name potassium inwardly-rectifying channel, subfamily J, member 12
Calculated molecular weight 433 aa, 49 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC027982
Gene symbol Kir2.2
Gene ID (NCBI) 3768
RRIDAB_2130478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Kir2.2 antibody 14135-1-APDownload protocol
FC protocol for Kir2.2 antibody 14135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols