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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using Kir7.1 Polyclonal antibody (12657-1-AP)

    WB analysis of human brain using 12657-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12657-1-AP (Kir7.1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using Kir7.1 Polyclonal antibody (12657-1-AP)

    WB analysis of human kidney using 12657-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12657-1-AP (Kir7.1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Kir7.1 Polyclonal antibody (12657-1-AP)

    IHC staining of human small intestine using 12657-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12657-1-AP (Kir7.1 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Kir7.1 Polyclonal antibody (12657-1-AP)

    IHC staining of human small intestine using 12657-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12657-1-AP (Kir7.1 antibody at dilution of 1:50 (under 40x lens).

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Kir7.1 Polyclonal antibody

Kir7.1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12657-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

KCNJ13, KIR1.4, Kir7.1, SVD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, human kidney tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

12657-1-AP targets Kir7.1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kir7.1 fusion protein Ag3327 相同性解析による交差性が予測される生物種
Full Name potassium inwardly-rectifying channel, subfamily J, member 13
Calculated molecular weight 360 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC037290
Gene Symbol Kir7.1
Gene ID (NCBI) 3769
RRIDAB_2130488
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60928
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kir7.1, encoded by KCNJ13 gene, is a member of the inwardly rectifying potassium channel family of proteins. Kir7.1 is a low-conductance channel. At mRNA level, Kir7.1 is predominantly expressed in small intestine and also expressed in stomach, kidney, and brain. Mutations in KCNJ13 gene are associated with snowflake vitreoretinal degeneration.

Protocols

Product Specific Protocols
WB protocol for Kir7.1 antibody 12657-1-APDownload protocol
IHC protocol for Kir7.1 antibody 12657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Acta Biochim Biophys Sin (Shanghai)

Identification of lipid metabolism-related genes as prognostic indicators in papillary thyroid cancer.

Authors - Shishuai Wen
View Article