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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Kir5.1 Polyclonal antibody

Kir5.1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12696-1-AP

Synonyms

KCNJ16, BIR9

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using Kir5.1 Polyclonal antibody (12696-1-AP)

    WB analysis of human brain using 12696-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12696-1-AP (Kir5.1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Kir5.1 Polyclonal antibody (12696-1-AP)

    WB analysis using 12696-1-AP

    HEK-293 cells(heated at 100 ° C, heated at 37℃) and Hela cells(heated at 100 ° C, heated at 37℃) lysates were subjected to SDS PAGE followed by western blot with 12696-1-AP (Kir5.1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12696-1-AP targets Kir5.1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kir5.1 fusion protein Ag3405 相同性解析による交差性が予測される生物種
Full Name potassium inwardly-rectifying channel, subfamily J, member 16
Calculated molecular weight 418 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC033038
Gene symbol Kir5.1
Gene ID (NCBI) 3773
RRIDAB_2130617
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Kir5.1 antibody 12696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols