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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Kir5.1 Polyclonal antibody

Kir5.1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12696-1-AP

Synonyms

KCNJ16, BIR9

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using Kir5.1 Polyclonal antibody (12696-1-AP)

    WB analysis of human brain using 12696-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12696-1-AP (Kir5.1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Kir5.1 Polyclonal antibody (12696-1-AP)

    WB analysis using 12696-1-AP

    HEK-293 cells(heated at 100 ° C, heated at 37℃) and Hela cells(heated at 100 ° C, heated at 37℃) lysates were subjected to SDS PAGE followed by western blot with 12696-1-AP (Kir5.1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12696-1-AP targets Kir5.1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kir5.1 fusion protein Ag3405 相同性解析による交差性が予測される生物種
Full Name potassium inwardly-rectifying channel, subfamily J, member 16
Calculated molecular weight 418 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC033038
Gene symbol Kir5.1
Gene ID (NCBI) 3773
RRIDAB_2130617
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kir5.1, also known as KCNJ16, is a member of the inwardly rectifying potassium (Kir) channel family, which plays crucial roles in various physiological processes. These channels are characterized by their ability to allow potassium ions to flow more freely into the cell than out, a property that is vital for maintaining the resting membrane potential and regulating excitability in cardiac, neuronal, and smooth muscle tissues. Kir5.1 is specifically noted for its unique expression patterns and functional roles in several tissues, particularly in the kidney and brain.

Protocols

Product Specific Protocols
WB protocol for Kir5.1 antibody 12696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols