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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCNMA1 Polyclonal antibody (22158-1-AP)

    WB analysis using 22158-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22158-1-AP (KCNMA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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KCNMA1 Polyclonal antibody

KCNMA1 Polyclonal Antibody for WB, ELISA
Cat No. 22158-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KCa1.1, K(VCA)alpha, hSlo, BKTM, BKCA alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain membrane protein, rat brain membrane protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22158-1-AP targets KCNMA1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNMA1 fusion protein Ag17802 相同性解析による交差性が予測される生物種
Full Name potassium large conductance calcium-activated channel, subfamily M, alpha member 1
Calculated molecular weight 1236 aa, 138 kDa
Observed molecular weight~130 kDa
GenBank accession numberBC137115
Gene Symbol KCNMA1
Gene ID (NCBI) 3778
RRIDAB_3669389
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12791
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calcium-activated potassium channel subunit alpha-1 also known as large conductance calcium-activated potassium channel, subfamily M, alpha member 1 (KCa1.1), or BK channel alpha subunit, is a voltage-gated potassium channel encoded by the KCNMA1 gene and characterized by their large conductance of potassium ions (K+) through cell membranes. KCNMA1 is widely distributed in human cell membranes, especially in muscle cells and neurons. They regulate the excitability of cells through sensitivity to calcium ions (Ca²⁺) and voltage, thereby affecting key physiological processes such as muscle contraction and nerve signal transduction.

Protocols

Product Specific Protocols
WB protocol for KCNMA1 antibody 22158-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols